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Absorption of omega-3 fatty acids by biomembrane models studied by differential scanning calorimetry

Maria Grazia Sarpietro a , Flavio Rocco b , Dorotea Micieli a , Maria Chiara Giuffrida a , Sara  Ottimo a , Francesco Castelli a,∗

a Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Viale  Andrea Doria 6, 95125 Catania, Italy

b Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, 10125 Torino, Italy

a b s t r a c t  

  

Omega-3 fatty acids, contained in vegetables and fish, exert several beneficial effects on the human health. The interaction of three omega-3 fatty acids (linolenic acid, docosapentaenoic acid and docosahexaenoic acid) with and their absorption by biomembrane models (represented by dimyristoylphosphatidylcholine multilamellar and unilamellar vesicles) were studied to get possible information on  how the absorption process of these compounds by  the cell  membranes after the intake of omega-3 fatty acids containing food could be. The differential scanning calorimetry technique was employed. The interaction of omega-3 fatty acids/vesicles was detected analysing phospholipid vesicles prepared in the presence of increasing amounts of the fatty acids. The  absorption kinetics of omega-3 fatty acids absorption by biomembranes was investigated setting pure phospholipid vesicles in contact with omega-3 fatty acids dissolved in the aqueous medium. Docosapentaenoic acid and docosahexaenoic acid interact with biomembrane models stronger than linolenic acid. In the aqueous medium the absorption of omega-3 fatty acids by unilamellar vesicles was faster than that by  multilamellar vesicles. The  omega-3 fatty acids are absorbed by  the biomembrane models gradually and almost completely. The  results suggest that the transfer of omega- 3 fatty acids from food to biomembranes could be  influenced by  the compounds structure and by  the medium in which they are dissolved.

© 2010 Elsevier B.V. All rights reserved.

Article history:
Received 16 December 2009
Received in revised form 10 March 2010
Accepted 11 March 2010
Available online 18 March 2010

Keywords:
Omega-3 fatty acids
LNA DPA DHA
Differential scanning calorimetry
Biomembrane models

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