Interaction of diethyl aniline methylphosphonate with DNA : Spectroscopic and isothermal titration calorimetry
Yan Lu n, Meihua Xu, Gongke Wang, Yun Zheng
College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, PR China
a r t i c l e i n f o
Article history:
Received 28 September 2010
Received in revised form
29 November 2010
Accepted 23 December 2010
Available online 1 January 2011
a b s t r a c t
In this study the diethyl aniline methylphosphonate (DAM) was synthesized, the interaction of DAM with ct-DNA has been investigated by fluorescence spectra, UV spectra, molecular modeling and isothermal titration calorimetry (ITC). The binding constant of DAM to ct-DNA calculated from both isothermal titration calorimetry and fluorescence spectra were found to be in the 104 M 1 range. According to the ethidium bromide displacement studies, UV spectra and isothermal titration calorimetry experimental results, it can be concluded that DAM is an intercalator that can slide into the G–C rich region of ct-DNA. Furthermore, the results obtained from molecular modeling corrobo- rated the experimental results obtanied from spectroscopic and ITC investigations. At the same time, fluorescence spectra suggested that the mechanism of the interaction of DAM to ct-DNA was a static enhancing type. ITC data showed that ct-DNA/DAM binding is enthalpy controlled.
& 2011 Elsevier B.V. All rights reserved.
Keywords: DAM, ct-DNA, Intercalation, Isothermal titration calorimetry, Molecular modeling
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